Protein powder being mixed inside a blender

Can You Mix Whey Protein the Night Before?

I know how busy everyday life can get for you, so you have no spare time to mix your supplements. This eventually goes on to negatively impact your nutrient uptake. 

As someone who gets incredibly busy, I’ve tried to find a solution, and one is to mix the previous night. 

My goal in this article is to expound on this method, primarily whether you can mix a popular supplement like whey protein the night before. 

Can You Mix Whey Protein the Night Before?

Yes, you can mix whey protein powder into a shake the night before. When mixed with water, milk, or your preferred liquid form, the powder may change its structure and texture and be left overnight. However, its nutritional value needs to be more balanced, as well as its safety and effectiveness. 

Ideally, you shouldn’t allow mixed protein powder to sit for more than 24 hours without taking suitable precautions, such as proper storage. While storing it in a refrigerator might help you preserve the taste for a more prolonged time period, other things must be considered.

Blue sneakers, jump rope, ball and a bag of protein powder placed on the floor

How to Mix Whey Protein the Night Before

Making protein shakes the night before is an excellent way of saving time and energy. When done correctly, pre-mixed protein powder can taste as fresh as freshly mixed one, offering you nutrition benefits and convenience. So, if you want to start mixing your protein earlier than needed, here’s how to do it right: 

Use High-Quality Protein Powder

Proteins come in various types, each with its defining characteristics. This protein has properties that make it break down faster than other proteins, such as casein for whey. Therefore, if you have mixed the powder overnight, consuming it as quickly as possible the next day is advisable. 

Also, the powder’s quality dramatically affects the shake’s quality. Therefore, opt for high-quality whey powder produced by reputable brands. This ensures that your mixed protein can stay fresh for longer. 

Mix in the Right Ingredients

When mixing protein powder to leave overnight, you need to be careful of the ingredients you use. Certain ingredients, when mixed with the powder and left overnight, may cause changes to its color, consistency, and taste. For instance, blending bananas or raw eggs into your premixed protein can give it an unpleasant color or taste by morning. 

Since low-quality ingredients automatically translate to subpar protein, here are some great ingredients to consider:

  • Fresh or frozen fruits
  • Milk or milk alternative
  • Vegetables
  • Nuts or nut butter
  • Ice cubes

Use The Right Storage Methods

When leaving mixed protein overnight, your storage method dramatically determines the protein quality you will get by morning. Therefore, do not leave the protein at room temperature overnight to maintain as much freshness as possible. Instead, do the following:

  • Store the protein in an airtight container to prevent contamination or oxidation. Glass containers are better options than plastic, as the latter retains smell and colors
  • Store it in a refrigerator to keep it cool and slow down bacteria growth. Protein shakes can last as long as 72 hours when refrigerated

If you refrigerate your protein overnight, some ingredients will have separated and settled by the morning. Therefore, you need to give it a nice, vigorous shake to mix the ingredients back together and restore its texture. 

Benefits of Mixing Protein Powder Earlier Than Needed

Mixing your whey the day or night before can provide you with the following benefits:

  • It helps you save time in the morning. With a protein shake ready to be consumed, you can simply grab one and go straight to other morning activities 
  • It affords you better control of your protein portions by allowing you to mix the exact amount of protein you will need for the next day 
  • It gives you the time and freedom to experiment with different ingredients combinations, flavors, and mixing styles. This is something you’re unlikely to do if you’re mixing your protein in the morning
  • Allows you to enjoy a cold shake in the morning  
  • Prevent lumpy shakes by giving the ingredients enough time to blend 

Drawbacks of Mixing Protein Powder Earlier Than Needed

Despite its many benefits, mixing your protein overnight may lead to the following unfavorable results: 

Man drinking his mixed protein powder from a plastic tumbler
  • It can reduce the freshness and taste of the protein shake 
  • It can cause the ingredients in the shake to settle and separate, leading to changes to the texture, consistency, and color of the protein shake
  • It increases the risks of spoilage and contamination, especially if not stored properly

How Long Can Whey Protein Shake Last in the Fridge?

Whey protein shakes can last as long as 72 hours while refrigerated. After this time, bacteria growth might begin, thus compromising the quality, taste, effectiveness, and safety of the shake. To keep your shake for a more extended period, consider freezing it. Protein shakes can last anywhere between 3 and 12 months when frozen. 

What Are the Risks of Mixing Protein Powder the Day Before?

One significant risk that comes with mixing protein the day before is spoilage. When the powder is mixed and left to sit at a warm temperature, bacteria grow, which could lead to spoilage. Other risks include changes in the protein’s texture, consistency, and taste, and contamination from other products.   

Do I Have to Drink My Protein Shake Immediately?

You do not have to drink your protein shake immediately after making it. You can always drink your protein shake hours or days after making it. Protein shakes can last about 3 or 4 hours at room temperature. To make it last for longer, store the shake in a fridge


It is safe to say that mixing whey protein the night before is a convenient and safe practice, and if done correctly, offers several benefits and nutritional value. To preserve the protein’s freshness and quality, place it in an airtight container and store it in a refrigerator. 

Jack Kelle

Jack is an entrepreneur, outdoorist, and animal lover with a background in philosophy, psychology, and business. He enjoys music, friends, and family. At RAVE, Jack works as the manager of marketing and content development.